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Objective:To evaluate the antioxidant activities and phytochemical content of the leaf and root extracts of Rumex crispus using the solvents extraction;methanol extract,ethanol extract,acetone extract (ACE),and water extract.Methods:Total flavonoids content,total phenolic content,and total proanthocyanidin were evaluated using spectrophotometric equivalents of the standards,quercetin,gallic acid and catechin respectively.The antioxidant activities of the plant extracts were determined using ABTS,DPPH,ferric reducing antioxidant power,total antioxidant capacity and nitric oxide scavenging assays.Results:The flavonoids and phenols contents of the extracts were in the range of (19.39 ± 4.08) to (526.23 ± 17.52) mg QE/g and (16.95 ± 12.03) to (240.68 ± 3.50) mg GAE/g,respectively.ACE of the leaf has the highest value of total flavonoids content (526.23 ± 17.52) mg QE/g while ACE of the root has the highest value of total phenolic content (240.68 ± 3.50) mg GAE/g.The highest content of total proanthocyanidin (645.38 ± 1.33) mg CE/g was in ACE of the root.Significant amounts of saponin and alkaloid were also present in the root and leaf extracts.All solvent fractions showed significant antioxidant activities (P ＜ 0.05) with ACE of the root having the highest scavenging value as shown in DPPH,ABTS,total antioxidant capacity,nitric oxide and ferric reducing antioxidant power (IC50 =0.014 mg/mL,＜0.005 mg/mL,0.048 mg/mL,0.067 mg/mL,and 0.075 mg/mL,respectively).Conclusions:In this study,the mean phytochemical content of the root of Rumex crispus is higher than that of the leaf and this may have contributed to its high antioxidant activities.This may also justify the frequent use of the root more than the leaves in traditional medicine for the cure of helminthic infections.
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Objective: To investigate phytochemical, antioxidant and antimicrobial activities of Kedrostis africana(K.africana). Methods: Dried tubers of K.africana were extracted in acetone,water and ethanol.The total phenol, flavonoid, proanthocyanidin and tannin contents were determined spectro-metrically. The antioxidant activity was examined using 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt, nitric oxide and hydrogen peroxide assays.The antimicrobial activity was determined by agar dilution method using minimum inhibitory concentration against 3 g positive and three gram negative strains while four fungal strains were also investigated. Results: Total phenol, flavonoids, proanthocyanidin and tannin contents ranged from (5.32 ± 0.01) to (10.51 ± 0.01) mg GAE/g; (42.58 ± 0.02) to (529.23 ± 0.01) mg QE/g;(15.05 ± 0.00) to (585.64 ± 0.00)mg CE/g and (0.301 ± 0.010) to (0.937 ± 0.000)mg TAE/g, respectively. The IC50values of the ethanol extract for 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulphonic acid)and hydrogen peroxide were 0.054 and 0.057 mg/mL, respectively,aqueous extract had an IC50value of 0.135 7 mg/mL for nitric oxide while the acetone extract had an IC50value of 0.300 mg/mL for 2,2-diphenyl-1-picrylhydrazyl. The ethanol extract demonstrated effective antimicrobial activity against the tested pathogenic species with minimum inhibitory concentrations values ranging from 2.5–5.0 mg/mL for bacteria and(0.312 5–5.000 0)mg/mL for fungi, respectively. Conclusions: The tuber of K. africana showed potent free radical scavenging property and antimicrobial activity.
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Objective:To investigate the antioxidant,antibacterial and phytochemical properties of ethanol extracts of Brachylaena elliptica and Brachylaena ilicifolia against wound infecting bacteria normally found in diabetic parents.Methods:The in vitro antioxidant activity of the two plants extracts were investigated spectrophotometrically using 1,1-diphenyl-2-picrylhydrazyl,nitric oxide,azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) dianunonium salt,hydrogen peroxide (H2O2) and ferric reducing power.The antibacterial assay and minimum inhibitory concentration (MIC) was determined using the agar dilution method against five bacteria strains using amoxycillin and ciprofloxacin as positive control.The phytochemical analyses (tannins,total phenol,flavonoids,flavonols,proanthocyanidin,alkaloids and saponins) were assessed using standard methods.Results:The ethanol extract of both plants exhibited strong antioxidant activities in some cases when compared to the standards (vitamin C and BHT).The antibacterial activity of both plants showed an appreciable broad spectrum activity against these wound pathogens with MIC value ranges between 0.3 mg/mL and 5 mg/mL.Tannins,phenols,flavanols,proanthocyanidins and alkaloids content of B.ilicifolia were significandy higher than those in B.elliptica.However,there were no significant differences in the flavanoid content of both plants extracts.Conclusions:These results indicated that the ethanol leaf extracts of these plants have antioxidant and antibacterial activity against the tested bacteria possibly due to the presence of bioactive compounds and therefore could be used as alternative therapy against wound infection caused by these bacteria in diabetic patients.
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Objective:To evaluate the nutritional composition and elemental constituents of Kedrostis africana and their safety aspect.Methods:Proximate parameters (moisture,ash,crude fibre,crude fat,proteins,and carbohydrate and energy) were evaluated using ALASA methods,and elemental analysis by ICP-OES technique.Results:The results from nutritional analysis showed that the tuber used for this study had a low content of crude fat and high content of ash,crude protein,crude fibre,carbohydrate and energy having the recommended dietary allowances.The tuber was rich in major minerals Na,K,Ca and Mg,there was sufficient amount of trace elements Fe,Cu,and Zn while the anti-nutrients oxalate,phytate,alkaloids,and saponins were detected in amounts that are not harmful according to Food and Agriculture Organization/World Health Organization.Conclusions:The outcome of this study suggests that this wild plant has very good nutritional potentials to meet the recommended dietary allowance and it could be a cheap source of essential nutrients that may ameliorate most nutritional challenges and can contribute remarkably to the amount of nutrient intake in human and animal diet.